Journal: Breast Cancer : Targets and Therapy

Article Title: CD151 Promotes Cancer Progression in Triple-Negative Breast Cancer by Inducing EMT through the MAPK Signaling Pathway

doi: 10.2147/BCTT.S518760

Figure Lengend Snippet: CD151 expression is up-regulated in TNBC tissues and cell lines. ( A ), Characteristics of patients with invasive breast cancer based on CD151 expression. Data are presented in percent stacted column chart. There were no significant differences found in lymph node involvement or the different stages of cancer, nor between ductal and lobular TNBC. Additionally, age did not appear to influence CD151 expression. Comparisons were made within groups. ( B ), TNBC samples were immunostained with anti-CD151 antibody. The figures illustrate representative samples of TNBC, adjacent tissue, and normal breast tissue. In normal breast tissue, CD151 was expressed in only a few cells. In the adjacent tissue, CD151 was expressed in the basal-myoepithelial cell layer of some cells. In invasive cancers, CD151 expression was primarily localized to the membrane, with some cells also showing expression in the cytoplasm. ( C ), CD151 mRNA and protein expression level in different breast cancer cell lines, data from the Proteinatlas database ( https://www.proteinatlas.org/ENSG00000177697-CD151/cell+line ). ( D ), CD151 mRNA expression in human breast cancer cell lines were analyzed by RT-qPCR, GAPDH as an internal reference (n=3). ( E ) CD151 protein expression in human breast cancer cell lines were analyzed by Western blot, GAPDH as an internal reference (n=3).

Article Snippet: Sections were incubated overnight at 4 °C with CD151 antibody (dilution ratio 1:200, Proteintech, 66567-1-Ig) followed by incubation with the corresponding biotinylated secondary antibody.

Techniques: Expressing, Membrane, Quantitative RT-PCR, Western Blot

Journal: Breast Cancer : Targets and Therapy

Article Title: CD151 Promotes Cancer Progression in Triple-Negative Breast Cancer by Inducing EMT through the MAPK Signaling Pathway

doi: 10.2147/BCTT.S518760

Figure Lengend Snippet: Knockdown of CD151 inhibits breast cancer cells migration and invasion but promotes proliferation in vitro via Epithelial–mesenchymal transition. ( A ), CD151 mRNA levels in HS578T cells which transfected with negative control (si-NC) and CD151 siRNA (si-CD151), GAPDH as an internal reference (n=3). ( B ), CCK-8 assay of cell relative proliferation in HS578T cells (n=5). ( C ), Representative images of transwell assay results of cell migration and invasion of HS578T cells (si-CD151 vs si-NC) (n=3). ( D ), Wound healing assay was performed to observe the role of CD151 in HS578T cells migration. ( E ), Western blot assay showed changes in EMT biomarkers’ expression in HS578T cells after CD151 knockdown. ( F-I ), Results of statistical analysis of protein expression of CD151, N-cadherin, Vimentin, and Snail. GAPDH was used as an internal reference for homogenization (si-CD151 vs si-NC) (n=3). Data are represented as the mean ± SD. * P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Article Snippet: Sections were incubated overnight at 4 °C with CD151 antibody (dilution ratio 1:200, Proteintech, 66567-1-Ig) followed by incubation with the corresponding biotinylated secondary antibody.

Techniques: Knockdown, Migration, In Vitro, Transfection, Negative Control, CCK-8 Assay, Transwell Assay, Wound Healing Assay, Western Blot, Expressing, Homogenization

Journal: Breast Cancer : Targets and Therapy

Article Title: CD151 Promotes Cancer Progression in Triple-Negative Breast Cancer by Inducing EMT through the MAPK Signaling Pathway

doi: 10.2147/BCTT.S518760

Figure Lengend Snippet: Overexpression of CD151 promotes breast cancer cells migration and invasion but inhibits proliferation in vitro via Epithelial–mesenchymal transition. ( A ), CD151 mRNA and protein levels in CD151-overexpressed HS578T cells, GAPDH as an internal reference (n=3). ( B ), CCK-8 assay of cell relative proliferation in HS578T cells (n=5). ( C ), Representative images of transwell assay results of cell migration and invasion of HS578T cells (CD151-overexpressed vs Vector) (n=3). ( D ), Wound healing assay was performed to observe the role of CD151 in HS578T cells migration. ( E ), Western blot assay showed changes in EMT biomarkers expression in HS578T cells after CD151 overexpressed. ( F-I ), Results of statistical analysis of protein expression of CD151, N-cadherin, Vimentin, and Snail. GAPDH was used as an internal reference for homogenization (CD151-overexpressed vs Vector) (n=3). Data are represented as the mean ± SD. * P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Article Snippet: Sections were incubated overnight at 4 °C with CD151 antibody (dilution ratio 1:200, Proteintech, 66567-1-Ig) followed by incubation with the corresponding biotinylated secondary antibody.

Techniques: Over Expression, Migration, In Vitro, CCK-8 Assay, Transwell Assay, Plasmid Preparation, Wound Healing Assay, Western Blot, Expressing, Homogenization

Journal: Breast Cancer : Targets and Therapy

Article Title: CD151 Promotes Cancer Progression in Triple-Negative Breast Cancer by Inducing EMT through the MAPK Signaling Pathway

doi: 10.2147/BCTT.S518760

Figure Lengend Snippet: CD151 is associated with MAPK and integrin signaling pathway and abnormal expression of CD151 disrupts MAPK signaling. ( A ), UMAP plot of 54836 cells colored by major cell type in this study. ( B ), Bubble plots of marker genes expressed in major cell types. Dot colors reflect expression levels and dot sizes represent the percentage of cells expressing marker genes in different cell types. ( C ), Pathway analysis was used to identify the significant pathway of the differential genes according to the KEGG, HALLMARK and GO database between C3 (TNBC) and C1 (Luminal ( B ) samples. ( D ), Pathway analysis was used to identify the significant pathway of the differential genes according to the KEGG, HALLMARK and GO database between C3 (TNBC) and C2 (Luminal ( A ) samples. We used the Wilcoxon test to select the significant pathways, and the threshold of significance was defined by p.adjust. ( E ), Western blot of CD151, JNK, p-JNK, ERK, p-ERK expression in CD151 knockdown or overexpressed cells when compared to control cells. GAPDH was used as an internal control. ( F-J ), Results of statistical analysis of protein expression of JNK, p-JNK, ERK, p-ERK. GAPDH was used as an internal reference for homogenization (si-CD151 vs si-NC) (CD151-overexpressed vs Vector) (n=3). Data are represented as the mean ± SD. * P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Sections were incubated overnight at 4 °C with CD151 antibody (dilution ratio 1:200, Proteintech, 66567-1-Ig) followed by incubation with the corresponding biotinylated secondary antibody.

Techniques: Expressing, Marker, Western Blot, Knockdown, Control, Homogenization, Plasmid Preparation

Journal: Breast Cancer : Targets and Therapy

Article Title: CD151 Promotes Cancer Progression in Triple-Negative Breast Cancer by Inducing EMT through the MAPK Signaling Pathway

doi: 10.2147/BCTT.S518760

Figure Lengend Snippet: CD151 can form a complex with integrin α3β1. ( A ), Co-immunoprecipitation of CD151 and integrins is shown. ( B ), Western blot of effect of CD151 expression levels on IGTA3 and IGTB1 expression levels, respectively. ( C ), Results of statistical analysis of protein expression of IGTA3 and IGTB1. GAPDH was used as an internal reference for homogenization (si-CD151 vs si-NC) (CD151-overexpressed vs Vector) (n=3). Data are represented as the mean ± SD. Significant differences compared with the control.

Article Snippet: Sections were incubated overnight at 4 °C with CD151 antibody (dilution ratio 1:200, Proteintech, 66567-1-Ig) followed by incubation with the corresponding biotinylated secondary antibody.

Techniques: Immunoprecipitation, Western Blot, Expressing, Homogenization, Plasmid Preparation, Control

Journal: Breast Cancer : Targets and Therapy

Article Title: CD151 Promotes Cancer Progression in Triple-Negative Breast Cancer by Inducing EMT through the MAPK Signaling Pathway

doi: 10.2147/BCTT.S518760

Figure Lengend Snippet: Impact of integrin α3 and β1 expression in CD151 overexpression HS578T cells. ( A ), CD151 overexpression HS578T cells were seeded into 6-well plates and then treated with specific siRNA for transwell assay, respectively. ( B ), CD151 overexpression HS578T cells were seeded into 6-well plates and then treated with specific siRNA for wound healing assay, respectively. ( C ), Western blot assay showed the effect of integrin α3 or β1 knockdown on the MAPK signaling pathway in CD151 overexpression cells. ( D-I ), Results of statistical analysis of protein expression of IGTA3, IGTB1, JNK, p-JNK, ERK, p-ERK. GAPDH was used as an internal reference for homogenization (Vector vs Vector+si-IGA3/si-IGB1) (CD151-overexpressed vs CD151-overexpresse+ si-IGA3/si-IGB1) (n=3). Data are represented as the mean ± SD. * P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: Sections were incubated overnight at 4 °C with CD151 antibody (dilution ratio 1:200, Proteintech, 66567-1-Ig) followed by incubation with the corresponding biotinylated secondary antibody.

Techniques: Expressing, Over Expression, Transwell Assay, Wound Healing Assay, Western Blot, Knockdown, Homogenization, Plasmid Preparation

Journal: Breast Cancer : Targets and Therapy

Article Title: CD151 Promotes Cancer Progression in Triple-Negative Breast Cancer by Inducing EMT through the MAPK Signaling Pathway

doi: 10.2147/BCTT.S518760

Figure Lengend Snippet: Schematic representation of the functional role of the CD151-integrin α3β1 complex in TNBC. In TNBC, CD151 forms a complex with integrin α3β1. Then, the complex activates the MAPK pathway, which promotes EMT and enhances the migration and invasion of TNBC cells.

Article Snippet: Sections were incubated overnight at 4 °C with CD151 antibody (dilution ratio 1:200, Proteintech, 66567-1-Ig) followed by incubation with the corresponding biotinylated secondary antibody.

Techniques: Functional Assay, Migration